Composite
Fre-L3-Stt

Part:BBa_K3887005

Designed by: Ruijuan Xiang   Group: iGEM21_BJU_China   (2021-10-01)


Fre-L3-SttH

Trp 6-halogenase for production of dihalogenated indigos

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 1282
    Illegal PstI site found at 1069
    Illegal PstI site found at 1168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 1282
    Illegal PstI site found at 1069
    Illegal PstI site found at 1168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 1282
    Illegal XhoI site found at 1813
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 1282
    Illegal PstI site found at 1069
    Illegal PstI site found at 1168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 910
    Illegal EcoRI site found at 1282
    Illegal PstI site found at 1069
    Illegal PstI site found at 1168
    Illegal NgoMIV site found at 942
    Illegal NgoMIV site found at 1200
    Illegal NgoMIV site found at 1903
    Illegal NgoMIV site found at 2169
    Illegal AgeI site found at 1088
    Illegal AgeI site found at 1429
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None